Branched Conjugates
Branched conjugates lift the constraint of classical 1:1 fusions, allowing any number of molecules to be joined at any chosen sites. They also enable non-canonical arrangements, such as head-to-head fusions.
Experimental data
Data in preparation
We are generating experimental data for this application. Ask us about our latest results.
How to use
We are still establishing this application. Tell us about your research, and we will work it out with you.
FAQ
Do I need a spacer between the tag and my protein?
For sterically demanding proteins, we recommend an extra arginine (R) N-terminal to the Lock-Tag and two extra alanines (AA) C-terminal to the Key-Tag. Many other conjugations work efficiently without any additional linker sequence.
Which buffers does Connectase work in?
Connectase works over a wide pH and salt range and tolerates common additives such as detergents and chaotropes. It has no special buffer requirements.
How long do I need to incubate?
Most reactions are finished after about one hour at room temperature. Higher temperatures speed up the reaction.
Do I need to remove the enzyme after the reaction?
In most cases, no. Connectase is used at low concentrations and catalyzes nothing beyond the conjugation. Where removal is needed, its His-tag allows capture on Ni-NTA resin.